Chapter 3. Substitution Patterns
Patterns of Substitutions withing Genes: Mutation rates, functional constraint, synonymous vs. nonsynonymous substitutions, Indels and pseudogenes, substitutions vs. mutations, fixation
Estimating substitution numbers: Juke-Cantor model, Kimura's two-parameter model, models with more parameters, substitutions between protein sequences
Variations in Evolutionary rates between genes
Molecular clock, relative rate test, causes of rate variation in lineages
Evolution in Organelles
Chapters 4. Distance-Based Methods of Phylogenetics
Advantages to molecular phylogenies
Phylogenetic trees and terminology: terminal nodes, internal nodes, inferred ancestors, Newick format, bifurcating, multifurcating, scaled trees, unscaled trees, rooted and unrooted trees (number of trees), Gene vs. Species trees, character and distance data, Pheneticists, and Cladists
Distance Matrix methods: UPGMA, estimation of branch lengths, transformed distance method,
and the general idea without the details of neighbor's relation method, neighbor-joining methods
Maximum likelihood approaches - general concept
Multiple sequence alignment and how it relates the phylogenetic analysis